BDP 581/591 is a bright and versatile fluorophore. It has a high brightness, significant two photon cross-section, and relatively long fluorescence lifetime. The dye reacts with reactive oxygen species (ROS) with the change of its fluorescence. It can be therefore used for intracellular monitoring of ROS. It can be used for the conjugation with both small molecules and biomolecules to construct tracers for fluorescence polarization assays and microscopy probes.

Providing storage is as stated on the product vial and the vial is kept tightly sealed, the product can be stored for up to 24 months.
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.