pHrodo-NHS is an NHS ester of a pH-sensitive fluorescent dye. The NHS ester at its terminus can covalently conjugate with the amine groups of proteins or peptides, enabling stable labeling. This dye exhibits pH sensitivity, showing weak fluorescence in neutral or alkaline environments but significantly enhanced fluorescence in acidic conditions (pH 4–6), making it suitable for dynamically monitoring acidic processes.

Providing storage is as stated on the product vial and the vial is kept tightly sealed, the product can be stored for up to 24 months.
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.