HCQ - 1 maleimide serves as an excellent building block for labeling thiol - containing molecules. The HCQ - 1 dye exhibits almost no fluorescence and has a moderate extinction coefficient. These dark quenchers are commonly utilized in molecular beacon probes. In such probes, the contribution of FRET to fluorescence quenching is extremely small. As a result, compared with other FRET probes like FRET peptides, the dependence on donor - acceptor spectral overlap is not as prominent. The HCQ - 1 dye is capable of quenching a variety of fluorophores, especially fluorescein derivatives such as FAM, HEX, TET, NED, and JOE.

Providing storage is as stated on the product vial and the vial is kept tightly sealed, the product can be stored for up to 24 months.
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.