5'- Biotin phosphoramidite serves as a useful tool for incorporating biotin into oligonucleotides. However, it has a specific limitation in that it can only be added to the 5'- end of the oligonucleotide chain. The DMTr (dimethoxytrityl) protecting group on the N1 position of biotin plays a crucial role during the coupling process. It effectively prevents the formation of branched structures, ensuring a more precise and controlled synthesis of the biotin - labeled oligonucleotides. Biotin itself possesses sufficient hydrophobicity. This property allows for a relatively straightforward separation between biotin - labeled oligomers (in the DMT OFF state) and unlabeled oligomers. Nevertheless, the DMTr group still has its unique value. It can be utilized to facilitate the purification process when using reverse - phase cartridges and high - performance liquid chromatography (HPLC). This additional assistance from the DMTr group can lead to more efficient and accurate purification of the desired biotin - labeled oligonucleotides.

Providing storage is as stated on the product vial and the vial is kept tightly sealed, the product can be stored for up to 24 months.
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.