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5'-Biotin CE Phosphoramidite

  Cat. No.:  DC67967   Featured
Chemical Structure
135137-87-0
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More than 5000 active chemicals with high quality for research!
Field of application
5'- Biotin phosphoramidite serves as a useful tool for incorporating biotin into oligonucleotides. However, it has a specific limitation in that it can only be added to the 5'- end of the oligonucleotide chain. The DMTr (dimethoxytrityl) protecting group on the N1 position of biotin plays a crucial role during the coupling process. It effectively prevents the formation of branched structures, ensuring a more precise and controlled synthesis of the biotin - labeled oligonucleotides. Biotin itself possesses sufficient hydrophobicity. This property allows for a relatively straightforward separation between biotin - labeled oligomers (in the DMT OFF state) and unlabeled oligomers. Nevertheless, the DMTr group still has its unique value. It can be utilized to facilitate the purification process when using reverse - phase cartridges and high - performance liquid chromatography (HPLC). This additional assistance from the DMTr group can lead to more efficient and accurate purification of the desired biotin - labeled oligonucleotides.
Cas No.: 135137-87-0
Chemical Name: 5'-Biotin CE Phosphoramidite
Synonyms: BIOTIN AMIDITE;[1-N-(4,4’-Dimethoxytrityl)biotinyl-6-aminohexyl]-2-(cyanoethyl-N,N-diisopropyl)phosphoramidite;5'-BIOTIN PHOSPHORAMIDITE;5′ Biotin Amidite;5′-Biotin Phosphoramidite;6-[(2-Cyanoethoxy)diisopropylaminophosphanoxy]-[1-N-(4,4′-dimethoxytrityl)biotinyl]aminohexane;DMT-Biotin-C6 Amidite;N-DMT-Biotinyl-2-Aminoethoxyethanol;DMT-Biotin-C6 Phosphoramidite;ST24044012;DMT-Biotin-C6 Phosphoramidite, configured for ABI;DMT-Biotin-C6 Phosphoramidite, configured for PerkinElmer, configured for Polygen;CID 91668436;PhosphoraMidous acid, bis(1-Methylethyl)-, 6-[[5-[(3aS,4S,6aR)-1-[bis(4-Methoxyphenyl)phenylMethyl]hexahydro-2- oxo-1H-thieno[3,4-d]iMidazol-4-yl]-1-oxopentyl]aMino]hexyl 2-cyanoethyl ester;[1-N-(4,4a€-Dimethoxytrityl)biotinyl-6-aminohexyl]-2-(cyanoethyl-N,N-diisopropyl)phosphoramidite;Phosphoramidous acid, N,N-bis(1-methylethyl)-, 6-[[5-[(3aS,4S,6aR)-1-[bis(4-methoxyphenyl)phenylmethyl]hexahydro-2-oxo-1H-thieno[3,4-d]imidazol-4-yl]-1-oxopentyl]amino]hexyl 2-cyanoethyl ester;DMT-BIOTIN- PHOSPHORAMIDITE;5'-Biotin CE Phosphoramidite;MFCD00797448;RBXYKQBFPLXSJS-RYQQVEDISA-N;6-(5-((3aS,4S,6aR)-1-(Bis(4-methoxyphenyl)(phenyl)methyl)-2-oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl)pentanamido)hexyl (2-cyanoethyl) diisopropylphosphoramidite;5-[(3aR,6S,6aS)-3-[bis(4-methoxyphenyl)-phenylmethyl]-2-oxo-3a,4,6,6a-tetrahydro-1H-thieno[3,4-d]imidazol-6-yl]-N-[6-[2-cyanoethoxy-[di(propan-2-yl)amino]phosphanyl]oxyhexyl]pentanamide;[1-N-(4,4'-Dimethoxytrityl)biotinyl-6-aminohexyl]-2-(cyanoethyl-N,N-diisopropyl)phosphoramidite;135137-87-0;1-N-(4,4'-Dimethoxytrityl)-biotinyl-6-aminohexyl]-2-cyanoethyl-(N,N-diisopropyl)-phosphoramidite
SMILES: S1C[C@H]2[C@@H]([C@@H]1CCCCC(NCCCCCCOP(N(C(C)C)C(C)C)OCCC#N)=O)NC(N2C(C1C=CC=CC=1)(C1C=CC(=CC=1)OC)C1C=CC(=CC=1)OC)=O
Formula: C46H64N5O6PS
M.Wt: 846.07
Purity: >98%
Sotrage: 2 years -20°C Powder, 2 weeks 4°C in DMSO, 6 months -80°C in DMSO
Cat. No. Product name Field of application
DC67979 ATO 532 ATO 532 Acid has an identical molecular structure to ATTO 532 Acid. It is a fluorescent label related to the well - known dye Rhodamine 6G. This dye is characterized by strong absorption, a high fluorescence quantum yield, excellent thermal and photostability, and good water solubility. Thanks to these features, ATO 532 is highly appropriate for single - molecule detection applications and high - resolution microscopy techniques, including Photoactivated Localization Microscopy (PALM), direct Stochastic Optical Reconstruction Microscopy (dSTORM), Structured Illumination Microscopy (SIM), and Stimulated Emission Depletion Microscopy (STED). In addition, it is also very suitable for use in Fluorescence - Activated Cell Sorting (FACS) and Fluorescence In Situ Hybridization (FISH). The fluorescence of ATO 532 Acid can be most efficiently excited within the range of 515 - 545 nanometers.
DC67978 ATO 488 Acid ATO 488 has the same molecular structure as ATTO 488. It is a new type of hydrophilic fluorescent dye that offers excellent water - solubility, strong absorption capacity, a high fluorescence quantum yield, as well as outstanding thermal and photostability. Due to these remarkable properties, ATO 488 is highly suitable for single - molecule detection applications and high - resolution microscopy techniques such as Structured Illumination Microscopy (SIM) and Stimulated Emission Depletion Microscopy (STED). Moreover, this dye is also well - suited for use in Fluorescence - Activated Cell Sorting (FACS) and Fluorescence In Situ Hybridization (FISH).
DC67977 ATO 465 NHS The ATO 465 molecule shares the same structure as ATTO 465. It is a fluorescent dye derived from the well - known acridone dye. ATO 465 has moderate hydrophilicity. Its fluorescence can be effectively excited within the range of 420 - 465 nm. This dye has remarkable features such as strong absorption, a high fluorescence quantum yield, excellent thermal stability, and high photostability. In an aqueous solution, this dye exhibits an astonishingly large Stokes shift of 55 nm. When it comes to a solid matrix, especially under low - temperature conditions, the dye emits intense and long - lasting phosphorescence.
DC67976 ATO 425 NHS ATTO 425 NHS ester is a new fluorescent marker based on the Rhodamine structure. It has strong absorption, high fluorescence quantum yield, high thermal stability and photochemical stability, and is suitable for single molecule detection and high-resolution microscopy. ATTO 425 NHS ester is an NHS ester derivative of ATTO 425 that can be used to label proteins or antibodies.
DC67975 ATTO 425 Acid The ATO 425 Acid molecule is equivalent to ATTO 425. It is a novel fluorescent marker with a coumarin structure. This marker boasts several notable characteristics, including a high fluorescence quantum yield, excellent photostability, and a relatively low molecular weight.
DC67974 AF488 Cadaverine AF 488 Cadaverine is a carbonyl - reactive structural unit. In the presence of activators like EDC or DCC, or activated esters such as NHS esters, it can be used to modify carboxyl groups through the formation of stable amide bonds. Additionally, it can serve as a polar tracer and an active dye for labeling proteins via carboxylic acid molecules.
DC67973 AF 647 NHS AF647-NHS ester is an analog of Alexa Fluor 647 (AF647). NHS ester can covalently bind to molecules with amino groups (such as proteins, antibodies, etc.). AF647 is a far-red fluorescent dye with an excitation wavelength (λex) of 635 nm (conventional fluorescence detection)/620 nm (instantaneous detection). Storage: Protect from light.
DC67972 AF 568 Acid AF 568 Acid shares the same chemical structure as Alexa Fluor 568 Acid. It is a bright red fluorescent dye that is optimally suited for use with the 568 - nanometer laser line. The AF 568 dye is soluble in water and is insensitive to pH values ranging from 4 to 10. AF 568 Acid represents the most cost - effective amine - reactive form for conjugating the Alexa Fluor 568 fluorophore with polypeptides.
DC67971 AF 488 Acid AF 488 is a vivid green fluorescent dye. Owing to its excellent photostability, it is frequently utilized in microscopy and cell detection. AF 488 can be used in conjunction with DAPI and is highly suitable for multiplex detection. It exhibits a high quantum yield and stable fluorescence within a pH range of 4 to 10.
DC67970 Biotin-dT Biotin - dT phosphoramidite contains a DMT (dimethoxytrityl) group, which enables quantitative coupling. It can be inserted into the target sequence as a substitute for the dT residue. This makes it an essential tool for adding biotin to the desired positions within an oligonucleotide sequence. It complements the commonly used biotin phosphoramidite. The latter lacks the DMT group and can only be added to the 5' end of the oligonucleotide, thus bringing the synthesis process to an end.
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