5'-Biotin CE Phosphoramidite Datasheet DC Chemicals
Description
5'- Biotin phosphoramidite serves as a useful tool for incorporating biotin into oligonucleotides. However, it has a specific limitation in that it can only be added to the 5'- end of the oligonucleotide chain.
The DMTr (dimethoxytrityl) protecting group on the N1 position of biotin plays a crucial role during the coupling process. It effectively prevents the formation of branched structures, ensuring a more precise and controlled synthesis of the biotin - labeled oligonucleotides.
Biotin itself possesses sufficient hydrophobicity. This property allows for a relatively straightforward separation between biotin - labeled oligomers (in the DMT OFF state) and unlabeled oligomers. Nevertheless, the DMTr group still has its unique value. It can be utilized to facilitate the purification process when using reverse - phase cartridges and high - performance liquid chromatography (HPLC). This additional assistance from the DMTr group can lead to more efficient and accurate purification of the desired biotin - labeled oligonucleotides.
