Timosaponin A-III|cas 41059-79-4|Supplied by DC Chem

Cas No.:	41059-79-4
SMILES:	C[C@]1([C@](C2)([H])[C@@](CC[C@@]3([H])[C@]4(C)CC[C@H](O[C@H](O[C@@H]5CO)[C@@H]([C@@H](O)[C@H]5O)O[C@@H]6O[C@H](CO)[C@@H](O)[C@H](O)[C@H]6O)C3)([H])[C@]4([H])CC1)[C@]7([H])[C@@]2([H])O[C@]8(CCC(C)CO8)[C@H]7C
Formula:	C₃₉H₆₄O₁₃
M.Wt:	740.92
Purity:	>98%
Sotrage:	4°C for 1 year, -20°C for more than 2 years

Description:	Timosaponin AIII could inhibit acetylcholinesterase (AChE) activity, with an IC50 of 35.4 μM.
Target:	IC50: 35.4 μM (AChE)[1].
In Vivo:	Of the tested steroidal saponins, Timosaponin AIII (TA3) most potently improves memory deficits. Timosaponin AIII increases the scopolamine-induced reduction in step-through latency by 17% (10 mg/kg), 28% (20 mg/kg), and 43% (40 mg/kg). During the acquisition trial, no differences in latent time are observed. Timosaponin AIII (20, 40 mg/kg, p.o.) potently inhibits this reduction of acetylcholine in scopolamine-treated mouse brain. The inhibitory effect of Timosaponin AIII is comparable to that of tacrine, which is used as a positive control[1]
In Vitro:	Timosaponin AIII could inhibit acetylcholinesterase (AChE) activity, with an IC50 of 35.4 μM[1]. Timosaponin AIII is identified as a major selective cytotoxic activity in BN108, and its selective cytotoxic activity involves inhibition of mTOR, induction of ER stress and protective autophagy[2].
Animal Administration:	Mice[1] Male ICR mice weighing 28-30 g are used. For the acquisition trial, mice are initially placed in the illuminated compartment and the door between the two compartments is opened 10 s later. Each group contains ten mice. One hour or 5 h before the acquisition trial, mice receive each test agent (e.g., Timosaponin AIII 10, 20 or 40 mg/kg, p.o. ). One hour before the acquisition trial, mice receive tacrine (10 mg/kg, p.o.) as a positive control. Memory impairment is induced by scopolamine treatment (1 mg/kg, i.p.) 0.5 h or 4.5 h after the administration of test agents, tacrine, or 10% Tween 80 solution. Control animals are administered 10% Tween 80 solution alone. Twenty-four hours after the acquisition trial, the mice are again placed in the illuminated compartment for retention trials. The time taken for a mouse to enter the dark compartment after the door opened is measured as the latency time in both acquisition and retention trials, with a maximum of 300 s[1].
References:	[1]. Lee B, et al. Timosaponin AIII, a saponin isolated from Anemarrhena asphodeloides, ameliorates learning and memory deficits in mice. Pharmacol Biochem Behav. 2009 Aug;93(2):121-7. [2]. King FW, et al. Timosaponin AIII is preferentially cytotoxic to tumor cells through inhibition of mTOR and induction of ER stress. PLoS One. 2009 Sep 30;4(9):e7283.

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