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Rhobo6

  Cat. No.:  DC67279   Featured
Chemical Structure
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More than 5000 active chemicals with high quality for research!
Field of application
Rhobo6 is a cell-impermeable small-molecule fluorophore designed for labeling the extracellular matrix (ECM) in live tissues. It contains a phenylboronic acid group that binds to diols commonly found in ECM glycans, resulting in a significant increase in fluorescence and a red shift in emission spectra. This property allows Rhobo6 to effectively visualize ECM architecture without perturbing native structures, making it suitable for long-term imaging studies. Additionally, Rhobo6's low affinity for monosaccharides enables reversible binding, which prevents photobleaching and allows for dynamic imaging of ECM components. While Rhobo6 does not specifically target individual ECM components, it provides a holistic view of ECM distribution and is particularly useful for studying ECM-related biological phenomena in samples that are not amenable to genetic manipulation or ex vivo culture.Rhobo6 is available under license from the Howard Hughes Medical Institute.
Chemicals PropertiesBiological activity Testing Data COA & MSDSRelated productsDatasheet
Cas No.:
Chemical Name: Rhobo6
Formula: C35H28B2N2O9
M.Wt: 643.2054
Purity: >98%
Sotrage: -80°C, desiccated, protected from light
Publication: Live imaging of the extracellular matrix with a glycan-binding fluorophore-Antonio Fiore, Guoqiang Yu, Jason J. Northey,Weaver & Kayvon Pedram -Nature Methods
Description: Rhobo6 is a fluorescent glycan-binding probe optimized for live-cell imaging and in vivo tracking of glycoconjugates. It exhibits pH-dependent binding (pKa ~5-7), high photostability, and minimal cytotoxicity. Compatible with mammalian cells, zebrafish, plant tissues, and mouse models.
In Vivo: 1- Inject 100 μL of 1 mM Rhobo6 (in 10% DMSO/PBS) intravenously. 2-Conduct imaging 30 min post-injection (optimal distribution).
In Vitro: Live-Cell Imaging (e.g., MCF10A)**​： 1 Wash cells with serum-free PBS.2 Add 5 μM Rhobo6 in serum-free media. Incubate 15 min at 37°C. 3 Replace with fresh media. Image immediately (avoid fixation). Tissue Staining (e.g., Mouse Pancreas)**​ 1 Perfuse tissue with PBS. 2 Incubate with 5 μM Rhobo6 in PBS (pH 7.4) for 30 min at RT. 3 Rinse 3× with PBS. Acquire images within 2 hours.
Animal Administration: 1- Inject 100 μL of 1 mM Rhobo6 (in 10% DMSO/PBS) intravenously. 2-Conduct imaging 30 min post-injection (optimal distribution).
References: Live imaging of the extracellular matrix with a glycan-binding fluorophore-Antonio Fiore, Guoqiang Yu, Jason J. Northey,Weaver & Kayvon Pedram -Nature Methods
Testing Data PDF
MSDS
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DC67279 Rhobo6 Rhobo6 is a cell-impermeable small-molecule fluorophore designed for labeling the extracellular matrix (ECM) in live tissues. It contains a phenylboronic acid group that binds to diols commonly found in ECM glycans, resulting in a significant increase in fluorescence and a red shift in emission spectra. This property allows Rhobo6 to effectively visualize ECM architecture without perturbing native structures, making it suitable for long-term imaging studies. Additionally, Rhobo6's low affinity for monosaccharides enables reversible binding, which prevents photobleaching and allows for dynamic imaging of ECM components. While Rhobo6 does not specifically target individual ECM components, it provides a holistic view of ECM distribution and is particularly useful for studying ECM-related biological phenomena in samples that are not amenable to genetic manipulation or ex vivo culture.Rhobo6 is available under license from the Howard Hughes Medical Institute.
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