Sulfo-Cyanine5 bis-NHS ester is a water soluble bifunctional dye possessing two NHS ester functions. It is an analog of Cy5? bis-NHS ester. The dye is useful for the cross-conjugation of amines, and for other advanced labeling applications.

JOE is a xanthene dye, fluorescein derivative possessing two chloro and two methoxy substituents. This fluorophore is a useful label for oligonucleotides. Its absorption and emission spectra are located between FAM and TAMRA. The fluorophore can be introduced into oligonucleotide using this phosphoramidite. It tolerates standard ammonium deblock conditions. This product contains pure isomer of 5-JOE dye.

Labeled deoxynucleoside triphosphates (dNTPs) provide an efficient way for the enzymatic labeling of DNA. DNA microarrays, PCR amplicons, and reverse transcripts can be labeled using various dNTPs. Sulfo-Cyanine3 is a dye emitting in yellow range. This fluorophore is a standard dye used in DNA microarray experiments. Sulfo-Cyanine3 dUTP is a labeled triphosphate providing efficient labeling in enzymatic reactions with various enzymes.

Alkyne maleimide is a bifunctional linker reagent which allows to attach terminal alkyne to various thiol-containing molecules, such as proteins containing cysteine residues. The alkyne moiety can be then conjugated with various azides via copper-catalyzed Click chemistry reaction.

Chloro-PEG3-azide is a heterobifunctional linker bearing a reactive chloride for the alkylation of various nucleophilic groups. Due to anchimeric assistance from oxygen, this atom is more reactive than in normal alkyl chlorides. The other reactive group is azide. It can be conjugated via Click chemistry, or reduced to amine group to be later acylated with reactive carbonyl compounds. PEG3 is a versatile hydrophilic linker for bioconjugation.

Sulfo-Cyanine7 is a near infrared, water soluble fluorophore. This derivative contains methyltetrazine group for the fast, efficient, and metal free conjugation with cycloalkenes in a reaction called TCO ligation.

TCO (tetrazine – trans-cyclooctene) ligation is one of the fastest reactions used for bioconjugation. It is an inverse electron demand [4+2] cycloaddition that takes place between tetrazine and trans-cyclooctene or other strained olefin. Sulfo-Cyanine5 tetrazine is a fluorophore derivative bearing a tetrazine group for the TCO-ligation based labeling. This reagent possesses good aqueous solubility and stability in biological environments.

PEG3 tetrazine is a monoreactive derivative that allows a facile attachment of hydrophilic triethyleneglycol fragment (pegylation) by inverse electron demand Diels-Alder reaction (ied-DA) with strained olefins, such as trans-cyclooctenes, and some cyclooctynes.

Fluorescein (FAM) is a popular fluorophore that has bright emission in the green area of visible spectrum. This derivative of fluorescein contains tetrazine moiety. Tetrazines react with trans-cycloalkenes and other strained olefins in a reaction called inverse electron demand Diels-Alder reaction (ied-DA). The reaction is very quick and specific. The reagent contains pure 6-isomer of FAM.

Tetrazine (methyltetrazine) derivative of fluorescein, pure 5-isomer. Tetrazines are reactive heterodiene components for [4+2] inverse electron demand Diels-Alder reaction (ied-DA) with strained cycloalkenes (trans-cyclooctenes, cyclopropenes). This reaction is also called TCO (tetrazine-cyclooctene) ligation. The reaction is among the fastest bioconjugation reactions

TCO ligation is a reaction that takes place between a tetrazine and a strained olefin, such as trans-cyclooctene. This tetrazine bears a fragment of near infrared dye, Cyanine7. This NIR fluorophore is especially useful for in vivo imaging.

Cyanine5.5 is a far red emitting dye for in vivo imaging and applications requiring low background. This Cyanine5.5 derivative contains tetrazine moiety that reacts with trans-cyclooctenes, cyclopropenes, and some strained cyclooctynes with the formation of stable conjugates. This labeling reaction is extremely fast, and can be used in vivo.

Tetrazine-cyclooctene (TCO) ligation is one of the fastest bioconjugation reactions known to date. In order to use it for fluorescent labeling, a fluorescent reporter molecule bearing one of the reacting groups is necessary. Cyanine5 tetrazine is a molecule bearing Cyanine5 fluorophore, and tetrazine moiety. Cyanine5 is a very popular fluorophore compatible with numerous instruments.

Cyanine3 tetrazine is a fluorescent dye with tetrazine moiety for a conjugation reaction called TCO (trans-cyclooctene) ligation. Actually, tetrazines react not only with trans-cyclooctenes, but also with (methyl)cyclopropenes and strained non-benzo-fused cycloalkynes. Cyanine3 is a moderately hydrophobic dye, enabling labeling in non-aqueous environment as well as in aqueous media containing some organic co-solvent like acetonitrile or DMSO

BDP TR dye tetrazine derivative for TCO ligation reaction of cycloalkenes with tetrazines. BDP TR is a multi-purpose borondipyrromethene dye for ROX/Texas Red channel, possessing excellent brightness.

BDP R6G is a borondipyrromethene dye that has absorption and emission wavelengths close to rhodamine 6G (R6G). BDP R6G is a very bright and photostable dye. Tetrazine fragment is used in inverse electron demand Diels Alder reaction with trans-cyclooctenes, acylazetines, and other strained olefins.

BDP 630/650 is a far red emitting, borondipyrromethene based fluorophore. The dye is tuned to match the standard Cy5 channel, and can be used as an alternative to Cyanine5 and sulfo-Cyanine5. Compared to cyanines, BDP 630/650 possesses a longer fluorescence lifetime which is important for fluorescence anisotropy measurements. BDP 630/650 has a brightness similar to cyanines, and an exceptional photostability. This amine derivative is useful for the reaction with electrophiles, and for enzymatic transamination labeling.

BDP 581/591 is a universal fluorophore with high brightness and photostability, relatively long fluorescence lifetime and two photon cross section. This derivative contains a primary amine group for the enzymatic transamination, and conjugation with electrophilic reagents.

Sulfo-Cyanine7 dicarboxylic acid (Сy7 analogue) is a water soluble, bifunctional NIR dye derivative bearing two carboxylic acid functional groups.

Cyanine7 dicarboxylic acid is a bifunctional dye molecule bearing two carboxylic groups. Cyanine7 possesses near infrared fluorescence.

This free carboxylic acid can be used as a non-reactive control in side-by side experiments with other reactive derivatives of BDP TMR. It can also be used for Steglich esterification.

BDP 630/650 is a bright far red fluorophore based on borondipyrromethene scaffold. This is a carboxylic acid derivative that can be activated for the coupling with amines. It can also be used as a control in negative experiments, or when chemical attachment of the dye is not necessary.

BDP 581/591 is a fluorophore that can be also used for ROS detection. This is a carboxylic acid dye derivative with a free COOH group.

BDP 558/568 is a universal borondipyrromethene dye with emission in the yellow region of the spectrum. This derivative is a free carboxylic acid.

Pyrene hydrazide is a carbonyl reactive dye for the attachment of pyrene polycyclic aromatic hydrocarbon residues to aldehydes and ketones. Pyrene is a blue-emitting fluorescent label which is especially useful as a proximity probe. When two pyrene residues are located close to each other, a longer-wavelength excimer fluorescence can be detected. Pyrene can also be FRET donor to other fluorophores. Its excited state possesses an unusually long lifetime of more than 100 ns, which allows to use this fluorophore in time-gated experiments.

Boc-protected Cyanine5 hydrazide reactive dye for the labeling of aldehydes and ketones. Boc- (tert-butoxycarbonyl) protective group can be removed with trifluoroacetic acid, or other strong acid. Reactive hydrazide is formed, which can then couple with various carbonyl groups yielding Cyanine5-labeled molecules.

Sulfo-Cyanine7.5 is a near infrared fluorophore with absorption and emission ranges around 800 nm. Its spectral properties allow for the efficient leveraging of tissue NIR transparency window. The fluorophore is available as different reactive forms, this one is maleimide for the labeling of SH groups of proteins and peptides. The sulfo- version is highly hydrophilic. Such labeled biomolecules are useful in biodistribution studies and visualization in intact organisms.

TAMRA (aka TMR or tetramethylrhodamine) is a xanthene dye that has been used as a fluorescent label for decades. Xanthene dyes are available as two isomers (called 5- and 6-isomers) that have almost identical fluorescent properties, but need to be separated to avoid doubling and smearing of labeled product peaks or bands during chromatography or electrophoresis. This is a pure 6-isomer of TAMRA maleimide, used for the labeling of proteins and peptides via thiol (SH) groups

Maleimide derivative of TAMRA (tetramethylrhodamine) for the labeling of thiol groups. Pure 5-isomer of TAMRA.

BDP TMR is a bright borondipyrromethene fluorophore for the TAMRA channel. This maleimide derivative is suitable for conjuction with thiol groups as e.g. in protein side chains.